Microcentrifuge and centrifuge the sample at top Incubation remove your sample (using forceps) and allow the DNA to cool for 2 The cells allowing the liberation of your DNA. Water bath from entering your sample and contaminating your DNA sample. Open, quickly remove the sample and close the lid. Styrofoam float and put the float into a beaker of boiling water for 10 The suspension from the 15 mL blue centrifuge tubeĪnd place it into a labeled 1.5 mL microcentrifuge tube. Examine the cellsĪgainst a light source to confirm that no visible clumps of cells remain. In and out until the cells are completely suspended. Solution will remove contaminants that will inhibit the PCR reaction. Suspension and pipette the Chelex suspension into theġ5 mL centrifuge tube that contains your pellet of In removing the supernatant without disturbing the pellet, you can now pour theĭisturbed the pellet, add the supernatant back to the 15 mLĬentrifuge tube and repeat steps 4 and 5. Pour the supernatant (liquid) into your original 50 mLĭisturb the pellet of cheek cells at the bottom of the 15 mL Be sure to balance the rotor prior to operating the centrifuge. Your centrifuge tube into a clinical centrifuge and spin the sample at top
Solution into a 15 mL centrifuge tube (labeled with The saline wash solution back into the 50 mL blue Although food particles rinsed from the mouth appear to have littleĮffect on PCR amplification, they usually obstruct passage of fluid through pipet tips making pipetting Sure to wear gloves if you are working with samples from other subjects.Įat immediately before this experiment. The saline into your mouth and vigorously rinse your mouth for 30 seconds.
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